![]() Such probes are widely used in a variety of assays, including RNA structure/function studies, Northern blot and primer extension analysis. MirVana Probe & Marker Kit provides reagents for the rapid preparation and purification of short, high specific activity 5' end-labeled RNA probes. Probes prepared with the kit are therefore suitable not only for solution hybridization assays, but also for Northern blot or in situ hybridization analysis (using nonisotopically labeled probes). A short sequence cleavable by RNase A and T1 is added to the 5' end of probe during the procedure. All you need to provide is an inexpensive, desalted, target-specific DNA oligonucleotide. MirVana miRNA Probe Construction Kit includes reagents for preparation of IVT templates as well as reagents for the IVT reaction. MirVana Probe & Marker Kit (5' end labeled probes). MirVana miRNA Probe Construction Kit (IVT probes) or MirVana miRNA Detection Kit using the indicated DNA or RNA probes included in the The expression of miR-16 miRNA and 5S rRNA was analyzed in 0.5, 1, 2 or 4 µg of FirstChoice Total RNA from mouse kidney included in the Comparison Between In Vitro Transcribed (IVT) and 5' End Labeled Probes. As a result, siRNAs and miRNAs have been primarily detected by Northern analysis, a technique that is time-consuming and relatively insensitive.įigure 4. RT-PCR, while being very sensitive, cannot be used to amplify small RNAs since in standard RT-PCR, the primers are about the same size as the small RNAs. A second technological challenge is that sensitive methods for the detection of small RNAs have not been previously available. Commercially available total RNA preparations do not always contain representative amounts of small RNA species because the RNA isolation protocols have not been optimized to recover small RNAs. For example, glass fiber filters, which are widely used to prepare total RNAs, do not quantitatively recover various RNAs that are smaller than 200 nt (such as 5S rRNA, snRNAs, snoRNAs, tRNAs, miRNAs, and siRNAs). Firstly, most standard RNA isolation protocols were not designed to efficiently recover small RNAs species. Successful Isolation & Accurate Measurement of miRNA expression from (FFPE) samplesĬurrently, the analysis of miRNA and siRNA expression suffers from two technological drawbacks.miRNAs cloned and sequenced from mouse, human, Drosophila, C.Isolating miRNAs from Pancreatic Samples.Stability of miRNA at Sub-Zero Temperatures.Sensitive Monitoring of microRNA Activity.Identification of Tropomyosin 1 as a Downstream Target of mir-21.Reagents for Up and Down-Regulation of miRNA Activity in Mammalian Cells.Small RNA Analysis - Rapidly Prepare Probes and Markers.Rapidly Optimize Anti-miR Inhibitor Transfection.Mouse RiboPure™-Blood RNA Isolation Kit. ![]()
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